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The aim of this study is to elucidate the genetic differences between the two species, and their subspecies, based on sequence analyses of nuclear DNA (first internal transcribed spacer of ribosomal RNA (ITS-1) and 18S rRNA) and mitochondrial DNA (16S rRNA and cytochrome oxidase I (CO1) gene). Based on the literature, five regions of the mitochondrial genome have been targeted: the cytochrome b gene, control region (D-Loop), NADH dehydrogenase 5 and cytochrome oxidase I (COI). Mitochondrial cytochrome oxidase I gene sequences support an Asian origin for the Portuguese oyster Crassostrea angulata. |
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