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DHPLC |
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According to the government's announcement the test is a "more accurate genetic testing method, denaturing high performance liquid chromatography, or DHPLC screening. For NQO1 exon4 and exon6, MPO promoter and exon 8, and CYP2E1 exon 6, the temperature of the DHPLC column was 63[degrees]C, 60[degrees]C, 60[degrees]C, 63[degrees]C, and 58[degrees]C; the acetonitrile gradient was 53-61%, 54-62%, 63-69%, 58-68%, and 52-58%, respectively. DHPLC applications screen and discover DNA sequence polymorphisms or changes in DNA sequences, including single nucleotide polymorphisms (SNPs). |
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