Clove bud essential oil had the highest DPPH
radical scavenging capacity of 285.
assay: this was conducted following the previously described DPPH
method (Amarowicz et al.
However, according to new research from the Department of Food Science at the University of Massachusetts, basing antioxidant activity claims on results of basic antioxidant assays such as ORAC and DPPH
could be misleading.
The amount of sample necessary to decrease the absorbance of DPPH
radical scavenging activity of oregano extract at the concentration of 1,000 [micro]g/mL was 28.
Similarly, the ability of phytic acid extracted from the cactus fruits in the present study to scavenge the stable synthetic DPPH
free radical was quite exceptional (86%).
The results of DPPH
assay (Table 1) showed increasing antioxidant activity with increasing of aqueous extracts concentration of wild and in vitro plants, it was observed a clear increase of RSA% at concentration 1 mg/ml and the highest value of RSA% was 85.
A 96-well microplate was used for the analysis where 140 [micro]L of the extracts of the individual herbs or the combinations were pipetted along with 60 [micro]L of 400 [micro]M DPPH
(prepared in the phosphate buffer solution).
Comparison of ABTS, DPPH
, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts.
The results are expressed in TEAC (Trolox Equivalent Antioxidant Capacity) applying the DPPH
The antioxidant activity of the extracts was assayed by measuring the DPPH
radical scavenging activity of the extracts.
Determination of Antioxidant activity DPPH
radical scavenging assay