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DTNB |
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For total GSH, GSH was oxidized by DTNB to give GSSG with stoichiometric formation of 5-thio-2-nitrobenzoic acid (TNB). One, a nonspecific agent called DTNB, blocks all enzymes at the cell surface, including PDI, that contain a hydrogen-sulfur group. Thiocholine then reacts with DTNB [Ellman's reagent, 5,5'-dithiobis (2-nitrobenzoic acid)] to produce a yellow color that is measured at 470 nm by the Test-Mate OP photometric analyzer (Ellman et al. |
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