ddNTP

AcronymDefinition
ddNTPDideoxynucleotide Triphosphate
ddNTPDideoxyribonucleotide triphosphate
References in periodicals archive ?
In a Sanger sequencing reaction, then, we end up with a mixture of polymerase extension products in which a very small fraction of the new DNA strands--all derived from the same template and starting location as defined by the primer--have selectively terminated at each base position along the template; and the terminating ddNTP identifies its template partner by the rules of hybridization mentioned above.
ddNTPs lack the hydroxyl (-OH) group on the sugar residue; this means that unmodified nucleotides (dNTPs) are unable to be added via the joining of the phosphate (P[O.
Also, minimum mass difference between heterozygotes increases to 16 Da for A/G heterozygote in SPC-SBE because of the use of biotinylated ddNTPs, compared with 9 Da for A/T heterozygote with regular ddNTPs.
1) Nonstandard abbreviations: HAEC, human aortic endothelial cell; HUVEC, human umbIIical vein endothelial cell; dNTP, deoxynucleoside triphosphate; ddNTP, dideoxynucleoside triphosphate; Ct, threshold cycle; s/n, signal-to-noise rafio; and s/i, signal iracfion.
Primer extension reactions were performed using the SNaPshot ddNTP Primer Extension Kit (Applied Biosystems, Foster City, CA, USA).
Primer extension reactions were conducted using the SNaPshot ddNTP Primer Extension Kit (Applied Biosystems, Foster City, CA, USA).
Primer extension reactions were performed with the SNaPshot ddNTP Primer extension Kit (Applied Biosystems).
The multiplex primer extension reaction was carried out with purified PCR products as template and a mixture of 12 site-specific primers (Eurogentec; Table 2), which were extended by one matching ddNTP (Amersham Bioscience).
The primer extension reactions were conducted with the SNaPshot ddNTP Primer Extension Kit (Applied Biosystems, Foster City, CA).
The 11 purified primers (Genosys; Table 1), each specific for a screened mutation, bind to the complementary sequence in the presence of fluorescently labeled dideoxynucleotide triphosphates (ddNTPs), and the AmpliTaq DNA Polymerase extends the primer by adding a single ddNTP to its 3' end.