| Acronym | Definition |
|---|
| DoS | Department Of State (United States) |
| DOS | Disk Operating System |
| DOS | Declaration of Security |
| DOS | Demokratska Opozicija Srbije (Democratic Opposition of Serbia; Coalition of Democratic Political Serbian Parties) |
| DOS | Dozing Off Soon |
| DOS | Direct Operating System |
| DOS | Dreaded Orange Spots (soapmaking, indicates soap has gone rancid) |
| DOS | Direction des Opérations de Secours |
| DOS | Dad Over Shoulder |
| DOS | Director Of Operations & Support |
| DOS | Directionally Oriented Structures |
| DOS | Date of Sobriety (recovery programs) |
| DOS | Denver Orchid Society |
| DOS | Date of Seeding |
| DOS | Deutscher Orgon Service |
| DoS | Daemons of Shadow (gaming) |
| DOS | Deployed Operational State |
| DOS | Denied Originating Service |
| DOS | Division of Operational Safety (US Atomic Energy Commission) |
| DOS | Dropout Studios |
| DOS | Diis Omnibus Sacrum (Latin: Sacred to All the Gods, epigraphy) |
| DOS | Designated Operator Service (SBC) |
| DOS | District Office Study |
| DOS | Data Overhead Switch (Nortel) |
| DOS | Densité d'Occupation des Sols (French: Density Land Use; France and Canada) |
| DOS | Detachable Output Stream |
| DOS | Director of Study/Studies |
| DOS | Duncan's Outdoor Shop (Bay City, Michigan) |
| DOS | Density of States |
| DOS | Day of Show |
| DOS | Department of Space (India) |
| DOS | Directly Operated Stores |
| DOS | Degree of Saturation |
| DOS | División de Organizaciones Sociales (Spanish: Division of Social Organizations; Chile) |
| DOS | Daughter of Satan |
| DOS | Dead on Scene |
| DOS | District Open Space (Hong Kong) |
| DOS | Director of Sales |
| DOS | Distribution of States |
| DOS | Deposition On Silicon |
| DOS | Digital Operating System |
| DOS | Distributed Operating System |
| DOS | Dependents Overseas |
| DOS | Date Of Separation |
| DOS | Denial Of Service (attack) |
| DOS | days of supply (US DoD) |
| DOS | Date of Service (health insurance) |
| DOS | Division of Safety (various locations) |
| DOS | Department of Surgery |
| DOS | Director of Emergency Services |
| DOS | Dosimeter |
| DOS | Degree of Superiority (algorithm comparison) |
| DOS | Distributed Object Server (Objectstream - SS7) |
| DOS | Director of Operations and Support |
| DOS | Dean of Students |
| DOS | Director of Security |
| DOS | Department of Sanitation (New York City) |
| DOS | Department of Statistics |