| Acronym | Definition |
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| DOT | Department Of Transportation | | DOT | Dictionary Of Occupational Titles | | DOT | Department of the Treasury | | DOT | Direction of Travel | | DOT | Department of Transport | | DOT | Department of Tourism | | DOT | Department of Trade | | DOT | Directly Observed Therapy | | DOT | Document Template (filename extension for Microsoft Word template) | | DOT | Department Of Telecommunications | | DOT | Director of Technology | | DOT | Damage Over Time (gaming) | | DOT | Deed of Trust | | DOT | Distributed Object Technology | | DOT | Delivered on Time | | DOT | Date of Termination | | DOT | Designated Order Turnaround (NYSE trading method) | | DOT | Director Of Training | | DOT | Digital Opportunity Task Force | | DOT | Days of Treatment | | DOT | Dynamic Overclocking Technology | | DOT | Duration Of Therapy | | DOT | Dutch Open Telescope | | DOT | Diffuse Optical Tomography | | DOT | Disodium Octaborate Tetrahydrate | | DOT | Diocese of Torit | | DOT | Design on Textile | | DOT | Date of Theft | | DOT | Druid of the Talon (video game character) | | DOT | Directory of Trades | | DOT | Designating Optical Tracker | | DOT | Digital Optical Tape | | DOT | Dynamic Object Technology | | DOT | Dose Optimized Thermotherapy | | DOT | Deployment Operations Team | | DOT | Digital Oligonucleotide Tiling | | DOT | Director On Target | | DOT | Division Officer Training | | DOT | Date of Transplanting | | DOT | Deep Ocean Technology/Transponder | | DOT | Distribution Oriented Technologies (Jordan, Amman) |
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