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DIG |
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Probes were generated by labeling cDNA, derived by reverse transcription of purified dsRNA with random hexanucleotide primers and digoxigenin (DIG)-11-dUTP. A reverse hybridization procedure was used to interrogate the macroarray, and hybridization was visualized by detecting the digoxigenin label colorimetrically according to the manufacturer's instructions (Roche). parahaemolyticus KXV237 strain (RIMD2210633) (8) as a template, the amplified DNA was labeled with digoxigenin using a PCR DIG labeling kit (Boehringer GmbH, Mannheim, Germany). |
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