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| Acronym | Definition |
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| DEF | Definition | | DEF | Definite | | DEF | Defrost | | DEF | Diesel Exhaust Fluid (automotive) | | DEF | Definitions (File Name Extension) | | DEF | Defend(ed) | | DEF | Deftones (band) | | DEF | Defense(ive) | | DEF | Defaults (File Name Extension) | | DEF | Dark Elf (Everquest) | | DEF | Diamond Empowerment Fund | | DEF | Danmarks Elektroniske Forskningsbibliotek | | DEF | Data Exchange Format | | DEF | Delayed Ettringite Formation (concrete) | | DEF | Design Exchange Format (Cadence) | | DEF | Decision Education Foundation | | DEF | Disarmed Enemy Forces | | DEF | Defense Enterprise Fund | | DEF | Drug Education Forum | | DEF | Deployment Expeditionary Force (US Army) | | DEF | Detector and Extractor of Fileprints | | DEF | Direct Equipment Failure | | DEF | Decayed, Extracted, and Filled Tooth | | DEF | Dose Effect Factor | | DEF | Digital Enforcement Faction | | DEF | Distance Enumerator Function | | DEF | Dependents Function | | DEF | Development & Evaluation Facility | | DEF | Digital Elimination Force | | DEF | Defensman/Defensmen (hockey) | | DEF | Detection Efficiency Function (microscope) | | DEF | Département de l'Emploi Francophone (French: Department of Employment Francophone; various locations) | | DEF | Diplôme d'Entraineur Fédéral de Football (French: Federal Football Coach Diploma) | | DEF | Détection Électronique Française (French: French Electronic Detection) | | DEF | Diplôme d'Etudes Françaises (French: Diploma of French Studies; various universities) | | DEF | Digital Empowerment Foundation (New Delhi, India) | | DEF | Diplôme d'Etudes Fondamentales (French: Fundamental Studies Diploma) |
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