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Antigenic analysis by direct immunofluorescence of 114 isolates of Rickettsia tsutsugamushi recovered from febrile patients in rural Malaysia. A punch biopsy of the tongue lesion identified chronic dermatitis; direct immunofluorescence revealed no immunoglobulin or complement deposition. Direct immunofluorescence is performed with nasopharyngeal aspirates to detect viral antigen on pharyngeal columnar epithelial cells by staining them directly with fluorescent antibody. |
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