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DTT |
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Briefly, we decondensed sperm heads on slides by incubation in 10 mM dithiothreitol (Saveen Werner AB, Malmo, Sweden)/0. 3), 25 mmol/L dithiothreitol (DTT), 20 mmol/L Zn[Cl. But by breaking the bond with reducing agents such as dithiothreitol, the scientists can reopen the active site and restore most of the enzyme's bactericidal activity. |
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