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| Acronym | Definition |
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| DO | Director of Operations | | DO | Data Object | | DO | Doctor of Osteopathy | | DO | Day Off | | DO | Data Out | | DO | Due Out | | DO | Delivery Order | | DO | District Office | | DO | Driver Only (train operated by the driver only) | | DO | Direct Object | | DO | Duty Officer | | DO | Digital Output | | DO | Design Overview | | DO | Development Officer | | DO | Director Officer | | DO | Dominican Republic | | DO | Disorder | | DO | Desk Officer (NYPD) | | DO | Dominica | | DO | Diário Oficial (Official Gazette; Brazil) | | DO | Distributed Object | | DO | District Officer | | DO | Dissolved Oxygen | | DO | Ditto | | DO | Division Officer | | DO | Dornier (German aircraft manufacturer) | | DO | Drawing Office | | DO | Doctor of Osteopathic Medicine | | DO | Detention Officer | | DO | Designated Official | | DO | Design Objective | | DO | Distributed Operations | | DO | Deportation Order | | DO | Denominacion de Origen (general identification for Spanish wines) | | DO | Disbursing Officer | | DO | Operations Directorate (NIMA) | | DO | Dispensing Optician | | DO | Directorate of Operations (US CIA) | | DO | Division Operator | | DO | Digital Orthophoto (FEMA) | | DO | Divisional Officer | | DO | Ombudsmannen mot Etnisk Diskriminering (the Swedish national ombudsman for issues on ethnic discrimination) | | DO | Diffractive Optics (camera lenses technology) | | DO | Determination Order (workers compensation claims) | | DO | Defense Order | | DO | Dommages Ouvrage (French: Work Damage) | | DO | Desktop Originator | | DO | Disciplined Oscillator | | DO | District Overseer (Jehovah's Witnesses) | | DO | Disbursement Order | | Do | Operational Dependability | | DO | Drift Orbit | | DO | DCS/Operations | | DO | Donk Out (poker) | | DO | Disbursing Order | | DO | Disto-Occlusal (dentistry) | | DO | Diode Outline | | DO | Distortion Offset | | DO | Dark One (Wheel of Time series) | | DO | Diatomic Orbital | | DO | Doctor of Otolaryngology | | DO | Defined Order | | DO | US Duty Officer | | DO | Daily Orange (Syracuse University student newspaper; New York) | | DO | Dawson and Oberman (plasmas) |
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