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| Acronym | Definition |
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| DFO | Department of Fisheries and Oceans (Canada) | | DFO | Designated Federal Official | | DFO | Disaster Field Office (US FEMA) | | DFO | Darkfall Online (online gaming) | | DFO | Deferoxamine | | DFO | Divisional Forest Officer | | DFO | Dairy Farmers of Ontario (Canada) | | DFO | Direct Factory Outlet (Australian retail center) | | DFO | District Forest Office | | DFO | Dartmouth Flood Observatory | | DFO | 1,8-Diazafluoren-9-One (forensic chemical) | | DFO | Director of Field Operations | | DFO | Done Fell Out | | DFO | Dislodged Foreign Object (gaming; World of Warcraft) | | DFO | Deutscher Falkenorden (German Falcon Order) | | DFO | Diesel Fuel Oil | | DFO | Dell Factory Outlet | | DFO | Digital Fuel Optimizer (RevTech motorcycle fuel control system) | | DFO | Done Fell Over | | DFO | Dining Facility Operation | | DFO | Distance from Origin | | DFO | Dungeon Fighter Online (video game) | | DFO | Divisional Finance Officer | | DFO | Documentation for Operations | | DFO | Duty Field Officer (UK) | | DFO | Dumb Freakin' Operator (polite form) | | DFO | Diploma in Front Office (various educational institutions) | | DFO | Des Filles en Ovalie (French: Girls in Rugby) | | DFO | Drilling Fluid Operator (offshore oil & gas) | | DFO | Distillate Fuel Oil | | DFO | Dolphins Fanatics Online (football website) |
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