may also increase the expression of IGF-1, an autocrine growth factor which accelerates cell proliferation in EBVaGC .
In situ hybridization for EBER was performed using a 30-base oligonucleotide complementary to a portion of EBER1
, a region of the EBV genome that is actively transcribed in latently infected cells.
All of the 10 smooth muscle biopsies were negative for EBER1.
The EBER1 hybridization demonstrated the absence of EBV in smooth muscle cells of all 10 cases of IHPS that were studied.
The nuclear signal for EBER1
mRNA was not detected by the in situ hybridization method.
In situ hybridization studies for Epstein-Barr virus (EBV) ribonucleic acid (RNA) were performed using a 30-base oligonucleotide probe complementary to a portion of the EBER1
gene and methods previously described by others.