The effects of RNAi are being studied in viruses such as EIAV, HIV-1, Hepatitis-C virus, Influenza-A virus, poliovirus, rotavirus, papillomavirus and respiratory syncytial virus with varying degrees of success reported (Saksela 2003).
This study utilized self-inactivating lentiviral vectors to establish transgenic fetal equine kidney (FEK) cell lines expressing shRNAs that target essential EIAV genes.
The initial goal of this research was to identify shRNA-expressing constructs that would render transgenic FEK cell lines resistant to EIAV infection.
The EIAV genes targeted in this study included gag, pol, S2, env and rev.
In addition to the EIAV targeting shRNAs, a transfer plasmid carrying a shRNA targeting the luciferase gene was also used to construct a lentiviral vector to generate a cell line to serve as the transgenic non-targeting control.
These cells served as the naive FEK cells for this and other studies of EIAV.
19], seven of which contained shRNA-expressing constructs designed to target essential EIAV genes and one shRNA-expressing construct targeted luciferase to serve as a transgenic control.
The primary means for EIAV entry into equine cells is via the ELR-1 receptor (Zhang et al.
They are derived from HIV and do not express the EIAV SU protein responsible for preventing EIAV superinfection.
Future studies utilizing RNAi to inhibit EIAV infection of naive cells could employ the use of superinfecting strains with the ability to infect these transgenic cells that are resistant to [EIAV.