This was done to minimize the time between the induction of signal transduction pathways and the exposure of these induced signaling pathways to the ELF-EMF.
4] cells per flask and exposed to ELF-EMF or sham conditions.
0 G (100 [micro]T) ELF-EMF resulted in a shift of the peaks to lower fluorescence intensities, thereby decreasing the ELF-EMF:sham ratio to [is less than] 1.
The inhibitory effect of ELF-EMF on the differentiation of Friend erythroleukemia cells was dose dependent (Table 2 and Figure 3).
Dose response of various field strengths at 60-Hz ELF-EMF on the differentiation of Friend erythroleukemia cells.
Induction of differentiation with DMSO caused a 100x decrease in telomerase activity in the sham-treated cells but only a 10x decrease in the ELF-EMF (1.
Driven by concerns raised by epidemiologic studies suggesting a potential cancer risk after exposures to ELF-EMF field strengths above the average exposure of 2 mG (0.
Our results, using two independent assays to measure hemoglobin content, demonstrated that inhibition of chemically induced hemoglobin-containing cells by ELF-EMF was dose dependent.
They saw no effect of ELF-EMF on the proliferation and differentiation of a Friend erythroleukemia cell line.
Because the potential risk of cancer has been a primary concern of ELF-EMF exposures, understanding both the mechanisms of carcinogenesis and the potential relevance of our in vitro system must be delineated.
It might seem that the use of an established erythroleukemia cell line and the results obtained from these cells would have no relevance to the potential mechanistic role that ELF-EMF might have in carcinogenesis.
In the absence of precise knowledge of the mechanisms by which DMSO induces Friend erythroleukemia cell differentiation, it is difficult to discuss the mechanisms of its inhibition by ELF-EMF.