To assess the potential activity of the 13 compounds as efflux pump inhibitors, a real-time EtBr
accumulation assay in a S.
iceren %2,5'lik agaroz jel elektroforezinde 110 volt'ta yurutuldu.
29) Efflux activity was assessed by means of a fast and practical test, the Ethidium Bromide-agar Cartwheel (EtBrCW), which provides information on the capacity of each isolate to extrude EtBr
from the cells by efflux, on the basis of the fluorescence emitted by cultures swabbed in EtBr
-containing agar plates.
Real-time PCR results showed that after 2 months of 50 ng/mL EtBr
treatment to deplete mtDNA, the ratio of mtDNA to nuclear DNA in [p.
5%, 3%, and 4% for samples 29, 17, and 20, respectively, which had not been detected by RFLP with EtBr
5 X TAE buffer stained with EtBr
at 100 V for 40 min.
Flow cytometry analysis clearly showed that CD significantly inhibited EtBr
efflux and extended post-antibiotic effect.
, DNA bands were visualized in a gel documentation system (DNR, Israel).
5% agarose gel in 1x TAE buffer containing EtBr
5 [micro]g/mL EtBr
bath and visualized using a UV transilluminator and AlphaImager 5.
Strains of the beta-lactamase positive and to be done antibiogram were incubated in LB broth containing 150 mg/mL EtBr
at 37[degrees]C for 24 h .
The final PCR products were electrophoresed on 2% agarose gels containing EtBr
along with DNA markers.