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EYFP |
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We used a chimera reporter fusion protein consisting of MT sandwiched between enhanced CFP (ECFP) and enhanced YFP (EYFP) molecules, and FRET between ECFP and EYFP was used to monitor conformational alterations in the core MT protein. Single fluorescent images of COS1 cells (n = 5) expressing Ca^sup 2+^/calmodulin-dependent protein kinase-type IIß fused with EYFP were analyzed using both the ID and 2D CWTs to compare their respective capacities to detect macrodomains that could be easily verified by visual inspection. Cg-TgN (Thyl-YFP)16Jrs mice (Jackson, distributed by Charles River, Wilmington, MA), which express EYFP at high levels in subsets of neurons, including the pyramidal cells of the hippocampus (31). |
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