To assess the potential activity of the 13 compounds as efflux pump inhibitors, a real-time EtBr
accumulation assay in a S.
iceren %2,5'lik agaroz jel elektroforezinde 110 volt'ta yurutuldu.
Real-time PCR results showed that after 2 months of 50 ng/mL EtBr
treatment to deplete mtDNA, the ratio of mtDNA to nuclear DNA in [p.
5%, 3%, and 4% for samples 29, 17, and 20, respectively, which had not been detected by RFLP with EtBr
Antibiotics and EtBr
were purchased from different sources, as follows: erythromycin, oxacillin, tetracycline and EtBr
from Sigma-Aldrich (Madrid, Spain) and ciprofloxacin from Fluka Chemie GmbH (Buchs, Switzerland).
In this connection, EtBr
mediated curing was performed (see Table 3).
The final PCR products were electrophoresed on 2% agarose gels containing EtBr
along with DNA markers.
For the HCV amplicon, when EtBr
was added, the peak height increased substantially and was accompanied by a slight increase in elution time.
stains DNA orange, which is mainly taken up by the cells when cell membrane integrity was lost.
5%) containing EtBr
was measured using a densitometer (Gel documentation system, EasyDoc, Korea).
The Polaroid Digital GelCam system allows users to customize their equipment to fit their needs -- offering packages that let the user choose a seven-inch or 11-inch hood, and either a 52mm 590nm EtBr
filter or optional 52mm 535nm SYBR green filter.
Should include 590/50 nm Orange filter for EtBr
, SYPRO Orange and