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All RT-PCR products were analyzed by electrophoresis on 2% agarose gels and visualized by staining with ethidium bromide. We detected amplification products using agarose gel electrophoresis and ethidium bromide staining. They electrophoresed for one and one-half hours, stained the gel with ethidium bromide for 10 minutes and viewed and photographed the Hhal fragments with an ultraviolet light source for the gel image photo. |
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