4 shows the nucleotide and amino acid sequences obtained using the Expasy
software for the metallothionein gene from Brassica rapa var parachinensis.
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Physicochemical parameters of a protein sequence were determined using Prot-Param through the ExPASy
6, based on ProtParam tool of ExPASy
Proteomics Server (29).
To classify identified proteins, 16 functional categories were established based on information from Gene Ontology database and additional information from ExPasy
The amino acid sequences were predicted with the 'Translate' program on the ExPASy
We also scored 10,000 random peptides of natural amino acid composition (25) derived from the ExPASy
(Expert Protein Analysis System) proteomics server at the Swiss Institute of Bioinformatics (Randseq, http://www.
The Swiss government, seeing the success of ExPASy
, said they would stop funding.
According to the ExPASy
Molecular Biology Server (17), the theoretical masses for the protonated wild-type [alpha]T-12 peptide and the Hb Groene Hart [alpha]T-12 peptide are 2967.
19) with the software accessible on the ExPASy