After that time, the samples were analyzed without further treatment by the ENZYMUN assays for F-PSA and T-PSA.
The ENZYMUN assays for F-PSA and T-PSA were performed with an ES 600 automatic analyzer from Roche Diagnostics GmbH as described by the supplier.
8 mL of the CaP plasma for 1 h to bind the F-PSA to the beads.
Similarly, F-PSA did not show any alteration of the HPLC peak after storage under similar conditions.
2, the PSA released from the complex was recognized in the ENZYMUN assays for F-PSA as well as for T-PSA.
1 mol/L ethanolamine for 60 h at different pH values, and the release of F-PSA was monitored using the ENZYMUN assays.
It can be seen that only a minor release of F-PSA is observed after incubation with 0.
The IMx assay has already shown ~50% lower responses to the PSA-ACT than to the F-PSA calibrators (12, 13, 15).
The T-PSA concentration measured by the equimolar assays is nearly equal to the sum obtained by the PA313/ FPA503 and [alpha]ACT/PA313 (F-PSA + C-PSA), whereas the T-PSA concentration measured by the IMx assay is higher than F-PSA + C-PSA.
1]-antichymotrypsin; CaP, prostate cancer; MAb, monoclonal antibody; F-PSA, free prostate-specific antigen; T-PSA, total prostate-specific antigen; ESI-LC/ MS, electron spray ionization mass spectrometry coupled with liquid chromatography; hK2, human glandular kallikrein; hK1, human pancreatic /renal glandular kallikrein; HRP, horseradish peroxidase; FIBS, 10 mmol/L HEPES, pH 7.
8 S42 78 ND ND PA313 71 ND ND (a) [PSA-ACT] was determined as F-PSA concentration (Materials and Methods); PSA-ACT was provided by Stanford University, Stanford, CA.