The testosterone levels measured in the FEGA are influenced by maternal factors, the intrinsic properties of the fetal testes, their age, the size of the explants, and the precise culture conditions.
Not only did this effort provide detailed dose-response data about many chemicals already known to be active in the FEGA (Ben Maamar et al.
Mixture ratios were set to reflect the potencies of all mixture components in the FEGA (Table 1).
Rather than studying every conceivable combination of chemicals within a mixture, the joint effects of anti-androgenic chemicals in the FEGA can now be approximated on the basis of the effects of each single component by using dose addition as the default assumption.
To utilize the FEGA in multi-component mixture studies required making a leap from qualitative studies to quantitative dose-response analyses.
A difficulty in using the FEGA as a screening method for the identification of chemicals with endocrine disruptive properties is the limited availability of human fetal tissue.
More studies using the FEGA are needed to establish these assumptions.
Future FEGA studies will help close this knowledge gap, especially if based on companion studies that identify all of the exogenous chemicals found in maternal and fetal tissues.
Once the allocation of the National Reserve has been made, the FEGA
has been able to verify that this award will not exceed the national limits established for direct aid in the community regulations, for which it has informed the Autonomous Communities that they can proceed to make the outstanding payment of 5% of the decoupled grants (basic payment scheme, green payment and supplementary aid to young people) corresponding to the 2017 campaign to all applicants for this aid.