FLOX


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AcronymDefinition
FLOXFluorine/Liquid Oxygen
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As shown in Figures 7(a)-7(d), immunoreactive protein levels of "active" cleaved caspase-1 and IL-1[beta] were significantly enhanced in the hippocampal CA1 region at 6 days after GCI in FLOX control mice, and E2 strongly suppressed the elevation of both of these factors.
As shown in Figure 8, FLOX control mice demonstrated a significantly increased NLRP3-ASC complex formation in the hippocampal CA1 region at 6 days after GCI as detected by the proximity ligation assay, and E2 treatment completely attenuated the complex formation.
As shown in Figures 9(a) and 9(b), triple immunofluorescence staining for the P2X7 receptor, Iba1 (microglia marker), and NeuN (neuronal marker) revealed that there was increased expression of the P2X7 receptor in the hippocampal CA1 region of FLOX control as well as PELP1 knockout mice after GCI.
a and c) Representative confocal images show that NLRP3 and ASC are induced in hippocampal CA1 region at day 6 after GCI in FLOX control as well as PELP1 knockout mice.
a and c) Representative confocal images show that cleaved caspase-1 and IL-1[beta] are induced in hippocampal CA1 region at day 6 after GCI in FLOX as well as PELP1 knockout mice.