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Fatty acid methyl esters were identified by comparing the retention time of experimental samples to standards. Derivitisation of NEFA and phospholipid fractions into fatty acid methyl esters was performed by adding 1 mL each of 14% (w/v) hexane and boron trifluoride : methanol reagent to the silica gel scrapings, and the tubes sealed firmly with Teflon tape, then heated at 100[degrees]C for 1 h. Fatty acid methyl esters were prepared by a direct transesterification reaction according to Lepage and Roy (1987). |
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