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5 mL of overlay medium that contained 3% medium viscosity carboxymethylcellulose prepared in MEM without phenol red with 10% heatinactivated fetal bovine serum, 1% glutamine (2 mmol/L), 100 U penicillin, and 100 [micro]g/mL streptomycin. 08 mg potassium chloride: residual components of MRC-5 (cloned, aborted-fetus) cells including (guinea pig) DNA and protein; and trace quantities of sodium phosphate monobasic, EDTA, neomycin, and fetal bovine serum. Aliquots of XTC-2 thawed from liquid nitrogen storage were seeded in culture flasks containing Leibovitz's L-15 medium supplemented with 10% fetal bovine serum (Sigma). |
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