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The aim of this study was to investigate the expression pattern of VEGF and flk-1 in three components (luminal epithelium, glandular epithelium and stroma) of human endometrium during different phases of menstrual cycle using tissue microarray (TMA) analysis.
Binding of VEGF or Flk-1 was detected by a biotinylated rabbit antimouse secondary antibody, followed by HRP conjugated avidin-biotin perioxidase.
Immunohistochemical labelling of VEGF, Flt-1, Flk-1, caspase-3, caspase-9, caspase-8 and Fas/FasL and TUNEL staining for detection of apoptotic cells were performed.
Circulating mature endothelial cells (CECs) may appear in the circulation by detaching from activated or damaged vessels and express CD34 and Flk-1, but, unlike the EPCs, they are negative for the haematopoietic marker CD45.
The number of cells positive for both Flk-1 and Sca-1 (Flk-1+/Sca-1+) and those positive for Sca-1 alone (Sca-1+) were measured by flow cytometry using an LSRII flow cytometer (BD BioSciences) and analyzed with FlowJo v8 software (Treestar Inc.
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