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Experimental cohorts were analyzed for dermo disease at deployment (time 0), approximately 2 and 4 wk, and at subsequent monthly intervals, using quantitative whole-body-burden alternative Ray's fluid thioglycollate medium (ARFTM) assays. Here, we describe an adaptation of Ray fluid thioglycollate medium (RFFM) assay to detect and enumerate Perkinsus spp. ABSTRACT Several molecular diagnostic assays have been developed in an attempt to replace the traditional Ray's Fluid Thioglycollate Medium (RFTM) assay for detection and quantification of Perkinsus marinus in oysters. |
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