Eight samples of influent (raw sewage, prior to treatment) and eight samples of effluent (post-treatment sewage) were analysed by conventional PCR for the presence of HAdV, EV, GARV and TTV, and by Colilert[R] commercial kit (Idexx Laboratories Inc.
The presence of total and fecal coliforms, as well as HAdV, EV, GARV and TTV was evaluated.
The authors detected HAdV in 100% of the samples collected at both raw and treated sewage, TTV in 72% and 62% of raw and treated sewage samples and GARV in 100% and 33% of tested influent and effluent samples, respectively.
The low rates for detection of GARV genomes may be associated to the methodology used for concentration of the samples, since other methods were used in the past for this purpose (Berthiaume et al.
As mentioned before, the results of the present study should be viewed with caution specially for the presence of GARV and TTV, with respect to: i) the initial volume used for the viral concentration, since there are studies that use more than 500 mL and viruses may have been diluted when discharged into water bodies; ii) the methodology used for the concentration of GARV, as there are studies (Berthiaume et al.
Fourteen water samples were analysed by conventional PCR for the presence of AdV, EV, GARV and TTV.
In the present study, the fecal contamination in surface water was evaluated by detection of AdV, EV, GARV and TTV.
Primers and conditions used for amplification of AdV, EV, GARV and TTV in PCR.