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Four micrograms of total RNA from each brain region were used as a template for reverse transcription-polymerase chain reaction (RT-PCR) with primer sets representing three regions of sequence conservation in flavivirus genomes: NS3-1 (EDL/Fla-U5004, 5'--GGA ACD TCM GGH TCN CCH AT and EDL/Fla-L5457, 5'--GTG AAR TGD GCY TCR TCC AT), NS5-1. |
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