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GF2

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AcronymDefinition
GF2Golgi Intermediate Fraction
GF2GeForce 2 (video card)


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PCR with Phusion High-Fidelity DNA Polymerase (Finnzymes, Espoo, Finland) was performed on extracted DNA (Wizard Genomic DNA Purification Kit; Promega, Madison, WI, USA) from the remaining crushed salivary gland suspensions (Bab primers GF2 and GR2, 540 bases long, variable part of the gene) (4) and from resulting parasitized erythrocytes (primers CryptoF and CryptoR, 1,727 bases long, complete gene) (12).
Acknowledgments We thank Norman Pieniazek for permission to use primers BAB GF2 and BAB GR2, Guy van Laere and his group for technical assistance in collecting roe deer samples, Bernard Bouet and Emmanuel Risi for efficient help in collecting fallow deer blood, Nadine Brisseau for technical assistance, and Richard Paul and Albert Agoulon for their critical reading of the manuscript.
 
 
 
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