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PCR with Phusion High-Fidelity DNA Polymerase (Finnzymes, Espoo, Finland) was performed on extracted DNA (Wizard Genomic DNA Purification Kit; Promega, Madison, WI, USA) from the remaining crushed salivary gland suspensions (Bab primers GF2 and GR2, 540 bases long, variable part of the gene) (4) and from resulting parasitized erythrocytes (primers CryptoF and CryptoR, 1,727 bases long, complete gene) (12). Acknowledgments We thank Norman Pieniazek for permission to use primers BAB GF2 and BAB GR2, Guy van Laere and his group for technical assistance in collecting roe deer samples, Bernard Bouet and Emmanuel Risi for efficient help in collecting fallow deer blood, Nadine Brisseau for technical assistance, and Richard Paul and Albert Agoulon for their critical reading of the manuscript. |
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Gezer Gezer gezh GeZi Gezira Gezira Gezira, Al GEZUB Gezuntheit Gezus Gezus Gezuz Gezuz gezv GF GF GF GF GF Formula GF Formula GF Formula GF Formula GF Handel GF Knap GF Knapp GF Napp GF&OD GF-BFSK gf-value GF/CM GF2 GF256GF3 GF4 GF8 GFA GFAA GFAAG GFAAS GFAB gFABP GFABS GFAC GFADS GFAE GFAE für angewandte Elektronik GFAF GFAFB GFAHA GFAI GFAK GFAL GFAM GFAP GFAP GFAP+ | |||||
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