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5%-2% agarose gels with GFX Columns (Amersham Biosciences, Piscataway, NJ, USA) according to the manufacturer's instructions. The Pico communicates through GFX proprietary Pico-Link, a proprietary protocol based on CANopen, that lets up to eight Pico units be connected from up to 1,000 m (3,280 ft) away, providing up to 272 I/O points through Pico expansion I/O modules. PCR products used for DNA sequencing were purified by using GFX PCR DNA and Gel Band purification kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA). |
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