We selected 18 candidate genes related to As metabolism (PNP, GSTO1, GSTM1, GSTP1, GSTT1, AS3MT, MTHFR, and CBS), oxidative stress (HMOX1, NOS3, SOD2, and CYBA), and inflammation/endothelial dysfunction (APOE, TNF, IL6, ICAM1, VCAM1, and S1PR1).
Other SNPs in genes involved in inflammation (APOE and IL6), oxidative stress (NOS3 and SOD2), and As metabolism (AS3MT, CBS, GSTO1, and MTHFR) showed nominally significant interactions with well-water As for associations with CVD, CHD, or stroke.
214142; National Center for Biotechnology Information 2007), GSTO1 (Hs.
The allele and genotype frequencies for the A222V (C[right arrow]T) MTHFR, E429A (A[right arrow]C) MTHFR, A140D (C[right arrow]A) GSTO1, and M287T (T[right arrow]C) AS3MT polymorphisms are shown in Table 2.
2003) observed that two individuals with an uncommon genotype of GSTO1 had an altered distribution of iAs metabolites in urine.
The genes of interest were selected by a) searching for new and previously reported polymorphisms in GSTO1 and AS3MT by sequencing; and b) screening for previously reported polymorphisms in GSTM1, GSTT1, GSTO1, MTR, and MTHFR.
Furthermore, samples taken year 2004 were genotyped for Glu155del (deletion of glutamate 155) and Thr217Asn in GSTO1 with RFLP.