Sequences of primers for real-time PCR Gene Primer Primer sequence (5'-3') ssc-miR-34c-5p RT-Primer CTC AACTGGTGTCGTGGAGTCGG CAATTCAGTTGAGGCAATCAG F-Primer CCCGCCAGGCAGTGTAGTTA R-Primer CTCAACTGGTGTCGTGGAGTC U6 F-Primer CTCGCTTCGGCAGCACA R/RT-Primer AACGCTTCACGAATTTGCGT ZNF148 F-Primer GCCTTTAGAACGAACTATCAC R-Primer AGGTACTTCTGTATGAAACGC KLF4 F-Primer TCGGACCACCTTGCCTTACA R-Primer TTGGGAACTTGACCATGATTGTAG PDGFRA F-Primer TCCAGCAGTTCTACCTTCATCA R-Primer AGGAGTCTATGCCAATGTCGTC GAPDH F-Primer GTTTGTGATGGGCGTGAAC R-Primer ATGGACCTGGGTCATGAGT PCR, polymerase chain reaction; zinc finger protein 148(ZNF148), kruppel-like factor 4(KLF4), platelet-derived growth factor receptor alpha (PDGFRA) and glyceraldehyde-3-phosphate
105 is inactive and 75 is active bands in ADAMTS5 Primary Primary Secondary Secondary antibodies antibodies antibodies antibodies concentration concentration Caspase-1 1/1000 Rabbit 1/4000 ADAMTS5 1/1000 Hare 1/4000 ADAMTS9 1/1000 Goat 1/4000 GAPDH 1/10000- Rabbit 1/4000 1/50000 Primary Reaction kDa antibodies Caspase-1 Mouse, rat, human 50 ADAMTS5 Mouse, rat, human 75/105 ADAMTS9 Mouse, rat, human 180 GAPDH Human 36 ADAMTS: A disintegrin-like and metalloproteinase with thrombospondin motifs; kDa: Kilodalton; GAPDH: Glyceraldehyde-3-phosphate
5) Human genes: TBP, TATA box binding protein; UBC, ubiquitin C; SDHA, succinate dehydrogenase complex, subunit A, flavoprotein (Fp); RLP13, receptor like protein 13; YHWAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; HMBS, hydroxymethylbilane synthase; B2M, fi-2 microglobin; hypoxanthine phosphoribosyltransferase 1; GAPDH, glyceraldehyde-3-phosphate
dehydrogenase; RPS18, ribosomal protein S18; ACTB, actin, beta; GSS, glutathione synthetase (hGus); 36B4, acidic ribosomal phosphoprotein P0.
2d3, treatment with tBHP resulted in a decrease in the levels of glycolytic enzymes (M2 splice isoform of pyruvate kinase, lactate dehydrogenase (LDHA) and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH)) in the H9c2 cells, and TPG effectively ameliorated this deregulated enzyme expression.
Enhanced expression of a glyceraldehyde-3-phosphate
dehydrogenase gene in human lung cancers.
We also quantified the transcripts of the housekeeping gene glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) as an endogenous control to normalize each sample using sense and antisense primers, 5' CCA TCA CCA TCT TCC AGG AGC 3' (positions 278-298) and 5' GGA TGA TGT TCT GGA GAG CC 3' (positions 663-682), respectively (Tokunaga et al.
F-actin has been shown to bind glycolytic enzymes like fructose-l,6- biphosphate aldolase (aldolase) and glyceraldehyde-3-phosphate
2006), the glyceraldehyde-3-phosphate
dehydrogenase (GAPD) gene (Proceedings of the National Academy of Sciences, Li et al.
dehydrogenase (GAPDH), was also evaluated for betulinic acid, olenaolic acid, ursolic acid.
4] Human genes: EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate
Nicotinamide may use other mechanisms to preserve cellular energy metabolism that could depend upon glycolytic metabolism mediated by glyceraldehyde-3-phosphate
dehydrogenase (Kaanders et al.
PCR primers for rat glyceraldehyde-3-phosphate
dehydrogenase (G3PDH), IL-1, IL-6, TNF-[Alpha], IL-2, and IFN-[Gamma] were purchased commercially from Clontech (Palo Alto, CA); PCR primers for rat IL-4 and IL-5 were synthesized by the core facility of the Human Studies Division at the U.