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Discher of the University of Pennsylvania in Philadelphia and his colleagues had a hunch that a stem cell's fate might depend on the texture of the growth medium that researchers use to raise the cells in the lab. 2] Nunc cell culture flasks (Nunc, Roskilde, Denmark) in standard growth medium (DMEM; Gibco BRL; supplemented with 2 mM glutamine, 1% nonessential amino acids, 5% heat-inactivated FBS, and phenol red as an indicator of pH). He uses an ordinary cactus soil mix, available in most nurseries, as a growth medium. |
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