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RNA was extracted from the resulting supernatant of the effluent sample and the total volume of diluted stool sample by binding to silica beads in the presence of guanidinium isothiocyanate (11). Blood was collected in tubes containing EDTA to prevent clotting and transferred into Fastubes (Endotell, Allschwill, Switzerland) containing guanidinium isothiocyanate in 0. The method is based on the use of guanidinium isothiocyanate and adsorption of the nucleic acids to silica particles. |
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