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HBA1Hemoglobin Alpha-Locus 1
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The Hb Groene Hart mutation in this patient was located on the intact HBA1 gene (genotype, -[[alpha].
Groene Hart], [alpha] chain encoded by the HBA1 allele carrying the C[right arrow]T mutation in codon 119; TFA, trifluoroacetic acid.
MCA does not require the analysis of a single-copy gene for reference because HBA1 and HBA2 serve as reference to each other.
2], leave intact one 3'UTR of either HBA2 or HBA1 (3); therefore, all of these can most likely be diagnosed by MCA.
All three primers will anneal to both the HBA1 and HBA2 PCR products and were designed to work optimally under the same cyclesequencing condition.