Subjects: A total of 165 TB patients (outpatients) and 133 healthy volunteers (age ranging from 20 to 55 yr) were selected randomly, in this study to investigate the role of HBHA in TB diagnosis.
PBMCs were cultured with HBHA (2 [micro]g/ml), phytohaemaglutinin (PHA 4 [micro]g/ml, positive control) and PBS (negative control) for four days (8).
After the optimal concentration of HBHA protein was determined, the serum from each patient of PTB or EPTB and healthy P+B- and P-B- subjects was tested by enzyme-linked immunosorbent assay (ELISA) (8).
nHBHA was used for detecting the HBHA specific antibody of PTB and EPTB subjects with a sensitivity of 90.
The results of the serodiagnostic tests showed that the patients with PTB and EPTB could develop the humoral response against HBHA.
The mycobacterium HBHA is a surface-expressed adhesion that can affect binding to host epithelial cells via a unique Lys-Pro-Ala-rich C-terminal region [1,7,15].