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HEPES |
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coli or yeast cells were grown to exponential phase, harvested, washed with transport buffer consisting of 75 mM HEPES, 0. 7) was measured in left ventricular and plantaris muscle whole homogenates diluted 1:200 (weight per volume) in extraction buffer of 20 mmol HEPES and 1 mmol EGTA. RNase Free Duplex Buffer (100mM KAc/30 mM HEPES pH 7. |
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