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The allantoic fluid was harvested 2 days after injection, and influenza A virus was detected by using hemagglutination assays with turkey erythrocytes. The 1 survivor of the case cluster (Brazil outbreak 1, patient 4) seroconverted (titer = 1:160) from an undetectable indirect hemagglutination assay (IHA) titer around the time of her infection. All serum samples were initially screened with a passive hemagglutination assay to detect immunoglobulin (Ig) G antibody against F1 antigen of Y. |
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