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IPTGInstitute of Petroleum Technology Gandhinagar (India)
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The creatinase expression was done in LB broth medium containing kanamycin (50 [micro]g/ml) at 37 [degrees]C with 1 mM IPTG for two different times, 4 hr and overnight.
The recombinant protein was induced by IPTG treatment as confirmed by SDS-PAGE (Fig.
coli, [16] then to express the katG gene, it was done the addition of IPTG inducer and cold shock treatment to the culture of recombinant.
The MCS of Blue script lies within the LacZ gene, which when expressed, in response to the presence of synthetic inducer IPTG and produces the enzyme b-galactosidase.
CUZD1 production was induced by IPTG (isopropylthio-j8-galactoside) induction, as previously described (11).
The transformed cells were spread on LB agar plates supplemented with X-gal (300 [micro]g/mL), IPTG (120 [micro]g/mL), and ampicillin (100 [micro]g/mL) and incubated at 37[degrees]C overnight.
According to these cases at the continuances of zymogene fermentation of saccharomyces cerevisiae with AAT Gen and the starting of inspiration during 3/4/5/6/7 hours after insemination with IPTG 0.
Then, the transformed cells were spread onto LB agar plates containing 100 (Mu) g/mL ampicillin, 33 (Mu) L 24 mg/ml IPTG and 40 (Mu) L 20 mg/ml X- gal and incubated at 37 oC for 16 h.
The transformed cells were spread on LB agar plates containing X-gal (300 ug/mL), IPTG (120 ug/mL) and ampicillin (100 ug/mL).