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Upstream of imiR-2, a 142-bp sequence shared 76% nucleotide identity with part of the insertion sequence IS2. Through the joint venture with Neusoft and integration with IS2 the Company anticipates 2007 revenues of $21 million and gross profits of $6 million. For amplification of IS6110 sequences, PCR analysis included the use of primers IS 1 (5'CCTGCGAGCGTAGGCG TCGG3') and IS2 (5'CTCGTCCAGCGCCGCTTCGG3'), designed to amplify a 123-bp fragment (nt 1,510-1,632) of the M. |
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