Comparison of morpho-agronomic traits versus RAPD and ISSR
markers in order to evaluate genetic diversity among Cuminum Cyminum L.
This research was conducted to estimate genetic diversity and to assess relationships among 32 accessions of tamarind using ISSR
markers for the basis of a breeding program in Ecuador.
KEYWORDS: Durum wheat accessions, genetic diversity, ISSR
Correlation detection between SCoT and ISSR
was not significant, but highly complementary, indicating that the combination of the two marker systems could avoid biases based on a single marker.
In this study, it was used multiple regression analysis to identify associations between ISSR
and RAPD markers with some agronomic traits in lemon balm ecotypes.
assessed by SSR and ISSR
markers in Southern Brazil.
bataticola obtained from geographically distinct regions (10 states) over a range of 19 host plant species using RAPD and ISSR
The results of PCR analysis using ISSR
molecular markers produced a total of 105 bands or loci, of which 92.
Compared to other nonspecific PCR based markers, such as RAPD (Random Amplified Polymorphic DNA), the ISSR
produce fragments with great reproducibility because they are more robust, since they show a larger anchoring surface and have higher annealing temperatures (REDDY et al.
Analysis of the ISSR
data was done with POPGENE version 1.
Based on the total of 107 polymorphic RAPD and ISSR
bands, a matrix of standardized covariates was calculated for the 24 samples (6 samples investigated in this study and 18 control samples for taxonomic classification) and used as input variable in a principal component analysis (PCA) conducted with GenAlEx.
As analises de ISSR
foram conduzidas por meio de 15 primers, selecionados com base no padrao de amplificacao inicial com 40 marcadores de ISSR