The ITS-1 and ITS-2
of rDNA nested PCR exhibited a specificity of 100% for the negative controls (0 positives/51 samples) as well as, for those samples (0/92) with other diagnosed respiratory infections (Table 3B).
The amplified fragments of ITS-2
of the 2 species are similar in size, making detection of the parental species and their [F.
80pb), el ITS-2
completo y la region 5' del gen 28S rRNA (aprox.
herreri sus longitudes de banda para el ITS-2
del ADN ribosomal, se encontro que la secuencia fue identica; debido a ello, se propone dos subespecies P.
sequences-based identification of Trichogramma species in South America.
Qualitative analysis of ITS projects Strategy Detail Disappointment Damage Success ITS-1 Yes Yes Yes No No ITS-2
Yes No No No Yes ITS-3 Yes Yes Yes Yes No ITS-4 Yes Yes Yes Yes No ITS-5 Yes Yes Yes Yes No ITS-6 Yes No No No Yes
Further characterization of the ITS-2
locus for subtyping was considered necessary.
Sponge species were identified using standard taxonomic methods and sequence analysis of the entire ITS-2
and partial 28S ribosomal DNA (rDNA) subunits.
Of the 528 bp, 256 bp are from ITS-1 and 272 bp from ITS-2
galloi Zucchi using sequences of the ITS-2
region of ribosomal DNA.
cameronensis located at ITS-1 (5'- TTgreater than GGCCCTTgreater than CCCTTgreater than CTGGCGG-3') and ITS-2
(5'- GAGGCGCAGCCCTTCACTT-3') while there is only a single region in L.
rDNA sequencing of Gnathostoma species (Nematoda) and the elucidation of the species causing human gnathostomiasis in the Americas.