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Comparison of lipopolysaccharide (LPS) antigens from the 2 strains by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and immunoblotting showed that they differed in O-repeating units (Figure, panel B). Similar amounts of wild-type and mutant AQP9s were detected in the yeast membranes by immunoblotting (data not shown). We performed immunoblotting using polyclonal rabbit antibodies against either GLUT 1 (lot A970212)([parallel][parallel]) or GLUT 4 protein (RALRGT, lot 819/4299). |
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