The KOU isolate displayed more similarities with the lineage II WN viruses (residues 131, 172, 199, and 210) when signature motifs were compared.
The amino acid substitution at 903 separates the Indian WN viruses (903S) from the WN and KUN viruses of both lineages (903T), instead grouping them with the Malaysian isolate and the KOU virus.
The MAb 10A1, produced to the KUN isolate OR393 (26), reacted specifically with the Australian KUN isolates in ELISA and did not react with the KUN isolate from Malaysia (MP502-66) nor with KOU virus or any of the lineage I or lineage II WN viruses (Table 4).
The results of the phylogenic analysis in this report clearly illustrate that the KUN, WN, and KOU viruses make up a closely related group of viruses, which can be further subdivided into several subgroups on the basis of genetic and antigenic data.
The virus from Malaysia, KUN MP502-66, and the African virus, KOU, pose a conundrum as to their relationship with the WN and KUN group of viruses.
The binding patterns of MAbs to KUN and WN isolates did not differentiate these viruses into the same phylogenetic lineages observed in the dendrograms, although they did support the sequencing results by identifying the Australian KUN viruses, the Malaysian KUN virus, and KOU virus as distinct antigenic groups.