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5 pL 10X manufacturer's reaction buffer, 2 pL lOmM dNTP, 2 [micro]L 10-[micro]M primer mix, 4 [micro]L DNA template (0.
Louis, MO) and stock solution of lOmM concentration was prepared in dimethyl sulfoxide (DMSO) (Sigma, St.
In addition to Red Dot, they include American Select, Green Dot, Herco and Blue Dot, the latter a great performer in the lOmm Auto.
0% and vinblastine [greater than or equal to] 96% were provided by the University Pharmacy of the Johannes Gutenberg University (Mainz, Germany) and dissolved in PBS (Invitrogen, Eggenstein, Germany) at a concentration of lOmM.
Nonencapsulated CF was removed by gel filtration on a Sephadex G-75 column (QC, Canada) using iso-osmotic buffer (lOOmM NaCl, lOmM HEPES, pH 7.
For PCNA and cleaved capase-3 staining, antigen retrieval was done in lOmM citrate buffer (pH 6.
1 mM phenylmethyl-sulfonyl fluoride, 1 mM sodium orthovanadate, lOmM NaF, 5mM leupeptin, and 10(xgml_1 aprotinin).
3] containing lOmM DTT for 15min at 56[degrees]C, in 10 mM [NH.
The extraction buffer was supplemented with 1 x cocktail protease inhibitors (Roche Applied Science), 1 mM phenyl-methylsulfonyl fluoride (PMSF), lOmM sodium fluoride and 1 mM sodium orthovanadate.
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- lomi ha’a mauli ola
- Lomi Lomi
- Lomi lomi massage
- Lomidze, Georgii
- Lomidze, Georgii Iosifovich
- Lomilomi massage
- Lominadze, Vissarion
- Lominadze, Vissarion Vissarionovich
- Lommen Health Sciences Library
- Lomnicki, Tadeusz
- Lomnický Štít
- Lomnicky Stit
- Lomolo Blood Bath Adventure
- Lomond Loch
- Lomond Roads Cycling Club
- Lomond, Loch
- Lomonosov (disambiguation)
- Lomonosov Current
- Lomonosov Moscow State University
- Lomonosov of Moscow
- Lomonosov Porcelain Factory
- Lomonosov Reflector
- Lomonosov Ridge
- Lomonosov Upland
- Lomonosov, Mikhail
- Lomonosov, Mikhail Vasilevich