5 pL 10X manufacturer's reaction buffer, 2 pL lOmM
dNTP, 2 [micro]L 10-[micro]M primer mix, 4 [micro]L DNA template (0.
Louis, MO) and stock solution of lOmM
concentration was prepared in dimethyl sulfoxide (DMSO) (Sigma, St.
In addition to Red Dot, they include American Select, Green Dot, Herco and Blue Dot, the latter a great performer in the lOmm
0% and vinblastine [greater than or equal to] 96% were provided by the University Pharmacy of the Johannes Gutenberg University (Mainz, Germany) and dissolved in PBS (Invitrogen, Eggenstein, Germany) at a concentration of lOmM
Nonencapsulated CF was removed by gel filtration on a Sephadex G-75 column (QC, Canada) using iso-osmotic buffer (lOOmM NaCl, lOmM
HEPES, pH 7.
For PCNA and cleaved capase-3 staining, antigen retrieval was done in lOmM
citrate buffer (pH 6.
1 mM phenylmethyl-sulfonyl fluoride, 1 mM sodium orthovanadate, lOmM
NaF, 5mM leupeptin, and 10(xgml_1 aprotinin).
3] containing lOmM
DTT for 15min at 56[degrees]C, in 10 mM [NH.
The extraction buffer was supplemented with 1 x cocktail protease inhibitors (Roche Applied Science), 1 mM phenyl-methylsulfonyl fluoride (PMSF), lOmM
sodium fluoride and 1 mM sodium orthovanadate.