5 pL 10X manufacturer's reaction buffer, 2 pL lOmM
dNTP, 2 [micro]L 10-[micro]M primer mix, 4 [micro]L DNA template (0.
In addition to Red Dot, they include American Select, Green Dot, Herco and Blue Dot, the latter a great performer in the lOmm
0% and vinblastine [greater than or equal to] 96% were provided by the University Pharmacy of the Johannes Gutenberg University (Mainz, Germany) and dissolved in PBS (Invitrogen, Eggenstein, Germany) at a concentration of lOmM
Nonencapsulated CF was removed by gel filtration on a Sephadex G-75 column (QC, Canada) using iso-osmotic buffer (lOOmM NaCl, lOmM
HEPES, pH 7.
For PCNA and cleaved capase-3 staining, antigen retrieval was done in lOmM
citrate buffer (pH 6.
1 mM phenylmethyl-sulfonyl fluoride, 1 mM sodium orthovanadate, lOmM
NaF, 5mM leupeptin, and 10(xgml_1 aprotinin).
3] containing lOmM
DTT for 15min at 56[degrees]C, in 10 mM [NH.
The extraction buffer was supplemented with 1 x cocktail protease inhibitors (Roche Applied Science), 1 mM phenyl-methylsulfonyl fluoride (PMSF), lOmM
sodium fluoride and 1 mM sodium orthovanadate.