were isolated from 42 preheated soil samples plated on Luria-Bertani
One mL of Luria-Bertani
broth was introduced into tubes 2 to 11.
Bacteria were grown in 250 ml Luria-Bertani
broth at 37[degrees]C for 19.
coli MG1655 mutants were grown in Luria-Bertani
broth until the midexponential phase and were infected with P1 phage at a multiplicity of infection of approximately 5.
There are times of course, when Trypticase Soy (TSB) or Luria-Bertani
(LB) broths may be the better media choice for a particular experiment.
For further analyses, bacteria were grown in Luria-Bertani
broth or on Luria-Bertani
agar plates containing 0.
for Cu,Zn-superoxide dismutase: preparation of periplasmic extracts--Periplasmic extracts from the bacterial isolates grown in Luria-Bertani
(LB) broth (Hi-Media, India) for 30 h were prepared by the lysozyme-EDTA method described by Battistoni and Rotilio (21), briefly 250 ml of 30 h culture was centrifuged (Sigma 3K-30, USA) at 6000 g for 15 min.
Escherichia coli JM109 cells expressing the plasmid pBluescript were grown to late log phase in Luria-Bertani
broth containing 100 mg/L ampicillin.
coli J53 as the recipient, which was isolated on meropenem (4 mg/L) containing Luria-Bertani
agar and MacConkey agar, meropenem (4 mg/L), and sodium azide (100 mg/L) (14).
Of these 173 frozen isolates, 170 were revived by streaking onto Luria-Bertani
agar and grown overnight at 37[degrees]C.
coli J53 transconjugants were selected on Luria-Bertani
plates containing sodium azide and meropenem (100 [micro]g/mL and 1 [micro]g/mL, respectively).
PCR amplification was carried out with PCR primers (20 pmol/L each per 50 [micro]L reaction) (Integrated DNA Technologies, Coralville, IA, USA) (Table) and 1 [micro]L genomic DNA (extracted from overnight Luria-Bertani
broth cultures according to PureGene DNA isolation kit instructions [Gentra Systems, Minneapolis, MN, USA] and dissolved in 50 [micro]L 10 mmol/L Tris, pH 8.