In contrast to these various misidentified viruses, the OBS6657 isolate clearly grouped with the prototype MAGV strain BeAr7272 and showed 100% (N), 97.
To obtain sufficient data to provide a reliable framework for analyzing the MAGV isolates in this study, and in light of their recently demonstrated potential as human pathogens, we further conducted full-genome sequence analysis for several CVV strains and for the closely related PLAV and TLAV, and for the human pathogenic FSV, which also has been described to be closely related to CVV (35).
This observation would appear to explain the close antigenic relationship of FSV to both CVV and MAGV by complement fixation but not plaque-reduction neutralization test, as well as the lack of sequence relationship between the S segments of these viruses.
One important consequence of the genetic reassignment of not only TLAV and PLAV, but also of MAGV (CoAr3363) to the CVV group, and identification of FSV as a previously unrecognized CVV reassortant, is to substantially expand the known geographic range of CVV.
Our analysis of the ORF and amino acid data identified several distinct clades representing CVV (lineages 1 and 2) and 2 separate clades of what had been identified as MAGV strains (Figure 2).
Surprisingly, a recent report showed that a virus isolate originally reported as MAGV was, in fact, a reassortant of Caraparu, a group C virus (4), which is genetically only distantly related to the Bunyamwera serogroup viruses.
In summary, we identified MAGV as the causative agent of a human febrile infection in Peru and showed that the virus associated with this infection is highly similar to the prototype MAGV isolate, suggesting that other viruses of this lineage also might have pathogenic potential in humans.
Geographic locations from which virus strains used in study of human infection with MAGV were isolated.